Platycodon grandiflorumhas long been used as a traditional oriental medicine for respiratory disorder. Platycodin D (PD) is known\nas the main component isolated from the root of PG. A simple and rapid liquid chromatography-tandem mass spectrometry (LCMS/\nMS) method has been developed and validated for the quantitation of PD in rat plasma. Quantitation was performed on a\ntriple quadrupole mass spectrometer employing electrospray ionization and multiple reaction monitoring in positive ion mode.\nThe total chromatographic run time was 4.0 min, and the calibration curves of PD were linear over the concentration range of\n50ââ?¬â??10,000 ng/mL in rat plasma. The coefficient of variation and relative error at five QC levels were 1.0 to 8.8% and 0.7 to 8.7%,\nrespectively.After a single oral administration of 500mg/kg and a single intravenous administration of 25mg/kg of 3% PD extract (a\nPG extract including 3% of PD), platycodin D and platycodin D3 were detected and pharmacokinetic parameters were estimated.\nThe oral bioavailability of platycodin D and platycodin D3 was 0.29% and 1.35% in rats at 500mg/kg of 3% PD extract of PG,\nrespectively.The present method can be applied to pharmacokinetic analysis of platycodins and platycosides of the PG.
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